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zkhan

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About zkhan

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  1. Based out of Georgia, USA. And I'll look into this. Thank you
  2. Ok, thanks for all your help so far. I'll look to purchase it outright. And thank you for letting me know the sigma tends to be overpriced, I had looked at the prices and assumed for them to be the norm. Could you suggest an alternative supplier to Sigma? I need to expose c. Elegans to an exogenous dosage of SAM in order to test other variables. I understand that SAM is already produced in c elegans, but I need an exogenous exposure for this project. And I'm hoping the constituents of a tablet or a capsule won't affect the amount of SAM the c. Elegans are exposed to, but that is a present concern of mine.
  3. Yes, this is a high school project. I'm currently a senior and this is my capstone project for the year. The lab I'm working in is a BSL-2 lab. And another reason for my desire to synthesize them in lab is because I'm not sure if I could totally purify them myself. So, I was hoping there might be a relatively clear and concise way to synthesize SAM via fermentation and then use the SAM synthesized in my project. This is what I've found online with regards to purchasing SAM outright and just using that in my experiment. Would you suggest these as a viable alternative, instead of synthesizing it? https://www.sigmaaldrich.com/catalog/papers/10762064 https://www.walmart.com/ip/Spring-Valley-SAMe-Enteric-Coated-Caplets-Value-Pack-400-mg-40-count/46336875?wmlspartner=wlpa&selectedSellerId=0&wl13=5275&adid=22222222228034717219&wl0=&wl1=g&wl2=m&wl3=76882799552&wl4=pla-149610495392&wl5=1015563&wl6=&wl7=&wl8=&wl9=pla&wl10=8175035&wl11=local&wl12=46336875&veh=sem&gclid=EAIaIQobChMIkYyY9p2Q5AIVIx6tBh2dlQvdEAQYCCABEgIvQ_D_BwE And I appreciate your concern regarding the academic integrity of this question, but I assure you, I'm simply trying to find SAM that I can use for my project. If pure SAM can be purchased outright, I'd be happy to. I'm just concerned about the purity of it. This is not to say that I'm oblivious to the fact that synthesizing SAM myself in lab would be difficult, but rather I'm just trying ensure that my project is up to the highest standards. Thank you
  4. Not necessarily, although it does venture a lot into chemistry. Could you provide me with a price estimate for the cost of the project and a link to the synthesis process you have in mind? I just want the SAM used in my project to be as pure as possible, I've found SAM tablets, but I'm concerned with how pure they are. Thank you again for your help so far
  5. I'm a student trying to produce it in lab for my capstone project. It will serve as one of the materials in my project. If you are familiar with any studies that clarify how to do so, I'd greatly appreciate it! :) Thank you
  6. Hello, all! I was wondering if there was anyway to produce s-adenosyl methionine in vitro? Links to studies or general advice would be greatly appreciated! Thank you
  7. I'm not able to view the replies. It says "Moderator Note Similar threads merged."
  8. I am a high school student working on an independent research project. How would I go about developing a custom antibody -it's sequence and structure- online? And if I were successful in doing that, how would I test the antigen's docking ability to the custom antibody? I understand the fact that I will need to apply for a license to use various computer software programs.
  9. I'm a high school student developing an independent research project. If something is off, please feel free to correct me; I need all the help I can got. Thank you First of all, could somebody please confirm that this is an antibody based off the mu-type opioid receptor? https://www.drugbank.ca/drugs/DB06317 If the antibody linked above is truly based off the mu type receptor, could I somehow alter its structure to bind to a specific antigen, such as heroin? How would I go about altering the structure, would I literally mess around with the structure or alter the amino acid sequence? How would I go about doing both of those things? Also, if somehow I am able to alter the antibody to become specific to heroin and treat heroin as an antigen, how would I go about testing the binding rate of heroin to this newly developed antibody? I would need some sort of data to show levels of affinity, what programs or algorithms would I use? Please feel free to add your own input; I really want to do this project, but I don't know how feasible this truly is. This is something I've become passionate about, so please, please help me if you can.
  10. Hi, I am a research student currently in high school. I am doing a project looking at the ability for antibodies to attack certain antigens. I need the amino acid sequence of the antibody for the drug heroin, if anyone is familiar with the sequence, please let me know or let me where I can attain that sequence. Once, I get that amino acid sequence, I need to be able to develop a 3D structure of that antibody. If anyone is familiar with an online program that does that, please let me know. I also need a somewhat simple computer program that I can use to determine the binding rate of an antigen to an antibody(in this case, heroin and its antibody). Please let me know if you are aware of any programs that let me do that. Thanks
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