PapainLCP
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caspase inhibitors + assays
PapainLCP replied to beachbum's topic in Biochemistry and Molecular Biology
Ok, I think that this is quite old so you might know by now. You can use an specific substrate with a warhead that binds covalently. There are several assays from luminescent/ fluorescent assays both in vivo and in vitro or commonly used fluotimetric peptides with tags that can be afterwards detected. -
How did you detect your gRNAs? Are you also using biolistics to avoid GMO regulation?
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Also looking for it since I had nice coments from a perso using it. The most similar one I have seen is here doi: 10.1038/nprot.2013.088 Cheers