dim2996

Optical density of what, the product of Seliwanoff's test? After adding Trichloroacetic acid, we also add 0.1% Resorcinol in 95% Ethanol and 30% hcl. Do you think that you could perform the same experiment in vivo as you do in vitro? Why or why not? Another thing to consider: if you stop glycolysis at the stage of producing fructose 1,6-bisphosphate, what will happen to the organism? As I understood our teacher said that at the moment when we add Trichloroacetic acid to cell we will destroy them. But we peformed experiment in such way: 1) isolation of enzymes from the cell mass; 2) carrying out the glycolysis reaction in vitro; 3) stopping the glycolysis reaction at the stage of fructose formation of 1,6-bisphosphate; 4) clarification of the filtrate; 5) determination of the concentration of fructose 1,6-bisphosphate as we understood our teacher asked why we cannot 1) carrying out the glycolysis reaction in vivo; 2) stopping the glycolysis reaction at the stage of fructose formation of 1,6-bisphosphate; 3) destroy cells; 4) clarification of the filtrate; 5) determination of the concentration of fructose 1,6-bisphosphate

I am a student. We made a laboratory work 'comparative analysis of the intensity of glycolysis in different microorganisms'. our teacher asked: why do we need to carry out the glycolysis reaction in vitro, and not in vivo?

by adding Trichloroacetic acid we measure optical density. we use Seliwanoff's test

My teacher said me that we can't compare the intensity of glycolysis if we will do experiment in vivo. We must do it only in vitro. Сomparative analysis of the intensity of glycolysis in different microorganisms was the theme of this work. We worked with Pseudomonas fluorescens Bacillus subtilis Candida scotti Saccharomyces cerevisiae In the our laboratory books: We must perform the process of glycolysis in vitro because it can be stopped at the stage of fructosodiphosphate formation. But our teacher said that we could stop the process in vivo also.

Sorry, my english is not good. I maybe make some mistakes(Maybe someone can answer this question:Why is the determination and comparison of the intensity of glycolysis in different microorganisms determined in vitro, and not in vivo?Conducting an in vivo test will not give accurate results.The determination takes place on an intermediate: fructosodiphosphate.Thank you