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just checking the formula for depth of coverage: no. of reads x read length/ genome size ??

Struggled to understand the difference between these paired-end: interleaved or separate data Interleaved: Reads of both ends of the sequencing run occur in one file. Read 2 of a pair directly follows read Read1 Separate: Forward and Reverse reads of a sequencing run occur in separate files. The order of reads in the file must be the same, i.e. the first read in the forward file and the first read in the reverse file are paired.