charmaine

Sorry. I'm very new to all of this.

What I am hoping to find out arethe problems that cause smearing of the bands on the agarose gel.

eck.

Thankyou kixxer!

Hey guys,

was wondering about the problems involved in PCR reactions on the agarose gel.

Was wondering what the problems were when you get smearing on the gel.

I have it listed as a problem with annealing temperature or mgcl concentration.

Are there any other reasons as to why you would get smearing?

thankyou

Hey guys,

was wondering if any one could give me or point me towards a simple explanation of the class switching in antibodies.

It seems that everything I look at on the web doesn't really explain it or how it happens.

thankyou!

I am getting a lot of samples from many different areas, then combining them all with the class.

*smiles* ok

Thanks guys for the help

Will post results when i have finished

jordan' date=' 17, present

 

why is first name required for this?[/quote']

 

So i can find out where every person on here lives, and stalk them.

Ok ok, It's just a part of the data that I have to enter for my assignment....

 

If you can add whether you are male or female, that would also help. (for those ambiguously nicknamed types)

haha ecoli. Any hair counts! I am just wondering how a pubic hair would come to be on ones hands...

I am doing a project on the mid phalangeal hair in humans.

I need to collect a load of data on the prescence of this hair.

If you have the spare time, could you please contribute! It will only take about 30seconds...

What you need to do is look at your hands, and see if you have a prescence of hair in the middle part of your fingers (not including thumbs)

I Have shown a picture on where to look.

If you have a prescence of hair here, Even ONE hair- Means that you have the allele.

Please include your first name, age and whether the hair is absent or present.

Thankyou

Have you even tried looking around for answers?

This thread is too long.

I am new.

It's all the same right?

Gram - have lipopolysaccharide

Gram + have peptidoglycan

Let's leave it at that...

Archae are the older ones... pretty much the ones that live in extreme conditions, eg halophiles, thermophiles, acidophiles etc... They are prokaryotes- lacking membrane bound organelles and they are unicellular

Eubac are the more complex ones. They are found pretty much everywhere, (humans, food etc) and aren't normally found in extreme conditions...

Protista are the ones that don't really fit into any other category... The 3 mains types are the protozoa (Animal like protists) Alagae (Plant like protists) and slime moulds (fungus like protists)

The grams stain is one of the most important stains in bacteriology/micro, etc. it pretty much divides bacteria into the 2 groups.

Gram positive is the purple/blueish dye, they retain the primary basic dye.

Gram negative happens when the species lose the primary dye and stain a pink/red.

The reaction of the bacteria- or colour to the gram stain is pretty much a reflection of the different chemical makeup of the cells of the two groups.

Gram positive obviously have a cell wall that contains fats/lipids talked about earlier.. (peptidoglycan)

Gram negative bacteria contains a layer of lipopolysaccharide which is absent in gram positive bacteria.

There are heaps of other ways to categorise bacteria.. This does not really group them into the pathogens/non-pathogens, it merely categorises the eubacteria into smaller groups.

-morphology/structural characteristics, growth, nutrition, biochemistry serology, genetics, ahh too many to type down- Are a few ways in which you can identify bacteria. The gram Staining is just one! (Of the broadest)

In order to find bacteria species and strains, you will have to use categorisation such as biochemistry, phage typing, and protein profiles....

*end rant*

One of the most used latissimus dorsi stretches is pretty much as follows.

-knees on floor

-hand over hand in front of you on the floor

-hips in air

-lean hips toward the side you wish to stretch...

You should feel the stretch from the shoulder to the armpit

At the moment I am doing a project on the allele for prescence of mid phalangeal hair..

What I have to do is pretty much collect a bunch of data, stick it in excel, and ramble for a few pages.

I would like a more thorough definition of what it is, but there doesn't seem to be much information around about it.

Obviously it is the prescence of hair in the middle joint of a persons fingers, and I see that it is dominant, but that is about all I can gather..

Does anyone know any more about this?