MissP.5_25

I get it now! Thank you!

Hi. I am doing some experiment on finding the electrical characteristics (surface conductance specifically) of a particle which is then attached to different amount of DNAs. The whole aim of this experiment is actually to analyse the sensitivity of this new found method of detecting DNA, but I don't think I need to explain it in detail for this particular problem. Now, this zeta potential is crucial because it's needed to calculate the crossover frequency of the DNA-attached particles. Crossover frequency is the frequency at which dielectrophoresis force is 0 (neither positive nor negative). After measuring the zeta potential of the particles, it was found that the zeta potential didn't show a significant change despite changing the ratio of particle to DNA. The crossover frequency however, did show a change corresponding to the different ratios of particle to DNA. Why is this so? And that's what I need to figure out. I am not so good at math so I am helping someone could solve this question, please. I guess the answer could be found by analysing this equation. The equation is attached.

There is a total of 8 wells and the the two ends of the wells are not used, meaning that only 6 wells are used in this experiment. The first four wells are filled with markers while the next two wells are are filled with DNA samples. Below are the amount of markers and DNA which are filled in their respective wells (in microL): M1 : 1 M2 : 3 M3 : 5 M4 : 10 DNA 1 : 3 DNA 2 : 5 This procedure is carried out to measure the concentration of DNA. Now, what I don't understand: 1. what is the function of the markers 2. why are four markers needed 3. how do you know how many microlitre of markers needed in each well (why 1, 3, 5 and 10) 4. why do you need two DNA samples when they're the same DNA, only different amount 5. how do you know how many microlitre of DNA to put in each well (why 3 and 5)