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newvital

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Everything posted by newvital

  1. The ratio of CD4 and CD8 is usually 2:1. In the spleen, there are about 20-30% of CD4+ T cells and 10-20% of CD8 T cells. In lymph nodes, it should be in that range, too, based on my impression.
  2. I actually bought the whole cholera toxin, including subunit A and B. Do I need to do the infection in a special containment facility?
  3. Thank you so much for the answer. I really appreciate it! So it should be OK for me to do this model in normal SPF facilities, right? It shouldn't spread to other mouse colonies, is that correct? Many thanks!!
  4. Hi all, this may be a naive question. I saw papers using cholera toxin to infect mice to induce antigen specific responses by T cells. Does anybody know if cholera toxin is dangerous or not? Does it infect human? How do I handle it? It's available from sigma, so I assume it shouldn't be dangerous. But just want to make sure. Thanks!!
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