DesmondP

I have been doing research on interactions between three membrane proteins in skeletal muscle. .I want to experimentally determine whether the interactions between these proteins are mutually exclusive or can occur simultaneously. Basically, protein a interacts with b, protein a interacts with c, and protein b interacts with c. I want to determine if these are individual heterodimeric interactions, or if a three-way complex can form. Most of what I have done is using whole muscle homogenates or transfected cells. Any ideas are would be helpful. Thanks! PFD