Ernesto1989
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Screening assay set up
Ernesto1989 replied to Ernesto1989's topic in Biochemistry and Molecular Biology
Ok,perfect. Thanks for your help! -
Screening assay set up
Ernesto1989 replied to Ernesto1989's topic in Biochemistry and Molecular Biology
Do you have experiences with a reverse phase array or something like a serum microarray? Thats what I would prefer to do, because we have already a spotting machine. Also papers about this topic would help... -
Hi people out there! I have to prove several serums of mice, if there are any antibodies (in the serum) against some choosed proteins. My question: How does such a screening assay looks like in the best case? Do you have experiences in that or did you maybe something similar? Please help me.. My approach would be to "stick" the proteins onto a nitrocellulose plate, let the serum flow over, wash and stain with a anti-mouse antibody to make those antibodies visible, which are still sticking.