Kriztin27 Posted June 22, 2016 Share Posted June 22, 2016 Hi everyone..how do we make a lipase standard curve of reaction rate vs time? I have 4-nitrophenyl decanoate as my substrate and other buffer and solution such as Tris-HCl, sodium taurocholate and NaCl. Link to comment Share on other sites More sharing options...
BabcockHall Posted June 23, 2016 Share Posted June 23, 2016 Your terminology is a bit unusual. A progress curve describes the rate of consumption of substrate or the appearance of product over time. A standard curve is sometimes created by making measurements of rate while varying the concentration of enzyme. Is this a homework problem or is this a research problem? Link to comment Share on other sites More sharing options...
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