northernlad2690

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About northernlad2690

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    Life science, Biology, Medical science
  1. i wonder if anyone could offer some subtle insight, I have a set of standards of substance X with an appropriate standard curve constructed from this info. The sample of unknown concentration is diluted 10X and thus has produced a much smaller absorbance reading as expected, but I'm stuck on how to correct for this dilution as i'm not sure whether to; A) multiply the absorbance reading by 10 or B) multiply by 10 the figure from the point on the graph where the diluted absorbance reading is.
  2. i wonder if anyone could offer some subtle insight, I have a set of standards of substance X with an appropriate standard curve constructed from this info. The sample of unknown concentration is diluted 10X and thus has produced a much smaller absorbance reading as expected, but I'm stuck on how to correct for this dilution as i'm not sure whether to; A) multiply the absorbance reading by 10 or B) multiply by 10 the figure from the point on the graph where the diluted absorbance reading is.
  3. thanks for the reply, I got there eventually.
  4. im assuming you are looking at Nickel affinity chromatography.
  5. What is the general principle of PCR-SSP? I get it uses sequence-specific primers to bind only to selected alleles but isn't this the principle in all PCR techniques?