Jump to content

endotoxin detection

Guest Kitty
Guest Kitty
in Biochemistry and Molecular Biology

Featured Replies

Hi , I work with a phosphorylcholine containing, tetrameric, N-linked glycoprotein (>200kDa) which is produced in, as close as possible, an endotoxin free environment however when we routinely test it using either a gel clot assay or a chromogenic microplate assay we get very inconsistent results eg adding polymixin to our sample has only a minor effect as does adding glucan blocker. Serial dilution of our sample only has a limited effect. We are presuming that this may have something to do with the structure of our molecule but are at a loss as to what this could be. Has anyone else experienced problems like these or even better has an explanation!

 

Thanks

 

Kitty

Archived

This topic is now archived and is closed to further replies.

Go to topic listing

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications
Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.