Jump to content

RBS on sequence of bacteria

Featured Replies

I have a genome sequence for a bacterial strain with an ORF that I'm sure encodes for a luxR gene (or similar), so a transcriptional regulator. I've also found the start and stop codon, but am having trouble finding the ribosome binding site (i.e. the Shine-Dalgarno sequence). I know typically it's AGGAGG, and that it does not always have to be so, but I'm unsure how 'different' it can be from the general consensus. Either way, I cannot seem to find a RBS. The closest I've managed to find is GAGAG, but I'm still uncertain about whether it could even be the RBS and am now starting to wonder if the ORF I have found even encodes for a real gene.

I also know that a RBS is only present if the gene encodes for a functional protein, but I can't find information on the gene encoding for rRNA or tRNA anyway.

Archived

This topic is now archived and is closed to further replies.

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications

Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.