Hi all,

have you ever tried to re-run an agarose gel after the run is finished and after visualizing it under UV? Last time I run a gel for a hour but the bands were not separate properly yet. I made the gel again but maybe (for a next time)it is possible to put the same gel on a second run after the analysis? Or does it get degraded?If yes, can you also explain me why?

Thank you very much.

Silvia

Yeah it's fine, the background will be stronger with the EtBr on the second run but if you just want to check for a band it is still useable. Maybe drop the exposure time a bit and see if it's more optimised.

Let the gel cool down a bit though, you don't want the gel to melt if it overheats.