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Biochemistry/enzymology for a follow up phone interview (drug discovery)

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I'm interviewing for an enzymologist position at a pharma company. So the keys here are models of inhibition and assay design.

Although I have some experience setting up kinetics assays, it was in a different context and with little/no support or criticism from anyone in my company. My questions are:

  • When would one choose an endpoint assay over a continuous/real time one?
  • In a kinase assay, what are some ADvantages of measuring ADP over the phosphorylated substrate?
  • When would you choose a linearized plot (eg Lineweaver Burke, Eadie Hofstee) over a non linear plot?
  • How would dose-response data on a semi-log vs. linear scale look?

Thanks in advance.

 

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