Jump to content

Microbiology question help

gouldbergvariations
in Biology

Featured Replies

I need a basic layout on how to go about answering this type of question.

 

You have available an Hfr lac- ala+ vitB+ tetS strain of E. coli and an F- lac+ ala- vitB- tetS strain.

You also have a P1 transducing phage and a tube of plasmids with a gene for tetracycline resistance. These are the only supplies you can use ( besides media and reagents). Describe how you can isolate a pure strain of E . coli lac+ ala + vitB+ tetR, where all genes of interest are present in the chromosome of the bacterium. Include gene transfer steps you would use, as well as the media ( m9 or Lb) you would use to select for successful gene transfers

 

Would appreciate any help as soon as possible, thanks

Archived

This topic is now archived and is closed to further replies.

Go to topic listing

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications
Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.