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question about differentation of THP-1

biomart
in Biochemistry and Molecular Biology

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In the lab I am working, THP-1 Cells differentiated by PMA to Macrophage cell just for 24 hour. That is:

we add PMA to THP-1 Cells and after 24 hour we change the media and without any delay we also treat the cells by our compound of interest to see changes in the cell signalling.

 

I have read in some papers that I should let the cells to be differentiated for 72hr before doing any next step.

 

what is you idea?

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