kixxer Posted March 10, 2005 Share Posted March 10, 2005 hi, I´ve had some problems with getting my E.coli expressed proteins endotoxin-free. There are commercial Kits with polymyxin- colums around, but when I use them not only the LPS, but the protein as well, gets bound to the column and I loose all my proteins. HAs anybody done this? greets, kix ________________________ http://www.biologia.fi Link to comment Share on other sites More sharing options...
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