Jump to content

Ways to Cleave FCP1 from GST Protein Tag

Robertmg
in Biochemistry and Molecular Biology

Featured Replies

Hello everyone,

 

In our lab we have been attempting to cleave FCP1 protein from its GST tag by using TEV protease. So far, several batches of TEV protease have been tried, but we have not gotten a significant amount of FCP1. I was just wondering if anyone had any suggestions or ideas for alternative methods for cleaving FCP1 from its GST tag that have produced good results. Thanks for any ideas.

Edited by Robertmg

Archived

This topic is now archived and is closed to further replies.

Go to topic listing

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications
Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.