Jump to content

Toxic Protein Expression in e.coli

RonSean
in Biochemistry and Molecular Biology

Featured Replies

I am currently trying to express a toxic Fab protein using e.coli. Unfortunately, I have to use this expression vector. I can successfully make the Fab protein at the 2ml scale but have not been able to express at larger 50-100ml scale. Details: pComb vector, SB media, Carbenicillan, with and without glucose, IPTG expression system (non T7 driven) and an e.coli strain that has the laq Iq gene to over express the lac repressor. I am a molecular biologist and am new to protein expression in e.coli. Any advice would be greatly appreciated.

  • 2 weeks later...

Have you tried altering time/temperature of induction to optimise it?

Also sometimes the concentration of inducer can affect things along with the type of media used.

Archived

This topic is now archived and is closed to further replies.

Go to topic listing

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications
Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.