Jump to content

Radioligand Binding Analysis

Peptides
in Homework Help

Featured Replies

Hi all, I have a question which I'm currently quite confused on.

 

To begin, a saturation binding assay on intact wild type AtnR cells using the tritiated AtnR angatonist [3H]DPPX has been performed. [3H]DPPX is added to cells in increasing concentrations in the presence or absence of cold competitor DPPX to determine the non-specific binding (NS) and total binding (TB), respectively. Each tube contains 15 cells in a final volume of 500µL. DPM (disintegrations per minute) results are obtained for each concentration.

 

From this, I must calculate the specific binding for each concentration.

 

To calculate the specific binding I'll first have to calculate the non-specific binding, and then minus this from the calculated total binding. However, I don't understand how to obtain these from the DPM results?

 

Any help on this would be much appreciated.

Archived

This topic is now archived and is closed to further replies.

Go to topic listing

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications
Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.