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Real-time PCR delay in Cq due to deletion SNP in primer

BarbaraBB
in Biochemistry and Molecular Biology

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I am collecting evidence, even anecdotal, how does single nucleotide deletion or insertion in primer region affect the outcome of real-time PCR. I am most interested in how much there is a delay in Cq (treshold cycle value) if compared to perfectly matched primer. But if you do not have Cq data, I also collect "Yes or No" answers, saying, whether there was amplification or no amplification.

 

I am interested specifically in insertion or deletion mismatches. (For substitution mismatches, there is a lot of data.)

I will appreciate any published or unpublished evidence.

Edited by BarbaraBB

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