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merodiploid

tomsmithucd123
in Homework Help

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Imagine you want to do some functional tests on some resistant mutants.

To aid you in this you use a set of old-fashioned F’ plasmids that carry various

parts of the chromosome

How could you construct a merodiploid strain that is heterozygous for your mutations

(sequenced above)?

Remember: every move you make requires a positive selection.

 

 

How could you determine whether your mutation is dominant or recessive?

 

 

 

What could you say about the protein whose alteration caused

resistance?

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