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phylogentic tree construction

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Hi,:)


I have isolated 9 soil bacteria and sent for sequencing. I had identified them using BLASTn. The results showed that 5 of them belong to genus Bacillus, 2 strains belong to genus Serratia , 1 strain is genus Pseudomonas and 1 strain belong to genus Escherichia.


Now, I would like to construct phylogenetic tree. However, I not sure how to do it.:confused:

So, can I just construct a phylogenetic tree that only consists 9 strains of my study bacteria samples?

Or do I need to construct a phylogenetic tree that consists of my study isolated bacteria together with some related bacteria species??


Please help me to remove all my doubt... Thanks.

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