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PEG silane layer and incubating slides??

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Hi everyone,

 

I am conducting a bio-chemistry related experiment and I have been unable to understand a step which is commonly performed.

My aim in this step is to apply a PEG silane layer.

 

 

 

After immersing ITO slides in a PEG-concentration, the slides are incubated for 18 hours at a temperature of 60 degrees Celsius.
Can you tell me why the incubation is performed? And why for 18 hours and at 60 degrees Celsius?
Thanks in advance.

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