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Enhancing the amount of bound protein to liposomes

Protein_Biochem1
in Biochemistry and Molecular Biology

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Hi Everyone,

 

I've been trying to develop a chloride-sensitive assay for an ion channel protein using a liposome system. The problem is that the equilibrium is shifted towards the free protein in solution rather than bound to the liposome.

 

Are there any methods/conditions that I could use to improve the amount of protein bound to the liposome? I'm currently using a 4:1:1 ratio of PC:PS:cholesterol for the liposomes prepared by extrusion and freeze-thaw

 

Thanks :)

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