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Sequencing Project

panayi1
in Genetics

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Given the sequence:

 

TCAGTTCAGCCCTCACCATTGCTCTGCAACTCAGAACAGTTACTACTGAGACTACCCTGAGAAGAGGATG

 

GTCCTAAACAGAGCTCTGATTCTGGGGGCCCTCACCCTGACCACCATGACAAGCCTCTGTGGAGGTGAAG

 

ACATTGTGGCGGACCACGTTGGCACTTATGGCACAGACTTCTACCAATCTCATGGTCCTTCTGGCCAGTA

 

CATCCACCAATTTGATGGAGACGAGGAGTTTTATGTGGACCTGGAGAAGGAAGAGGCTGTCTGGAGGCTG

 

CCTATGTTTGATAAATTACGTTTTCACCCGCAAGGTGCACTGAGAAACATAGCTATAGCGAAACACAACT

 

TGGATGTTCTGACAAAACTCTACAACTTTACCCCTGTTATCAATGAGGTTCCAGAGGTGACTGTGTTTTC

 

CAAGTCTCCCGTGATGCTGAGTCAGCCCAATACCCTCATCTGTCACGTGGACAACATCTTTCCCCCTGTG

 

ATCAACATCACATGGTTGAGAAACGGGCACTCTGTCACAGAGCATGTTTCTGAGACCAGCTTCCTCCTCA

 

GAAGTGATTATTCCTACCTCAAGATAAGTTATCTCCCCTTCCGCCCTTCTGATGATGATGTTTATGACTG

 

CAAAGTGGAGCACTGGGGCCTGGATGAGCCACTGCTCAAACACTGGGAGCCTGAAATTCCAGCCCCTATG

 

TCAGAGCTGACAGAGACTGTGGTCTGTGCCCTGGGGTTGACCGTGGGCCTCGTGGGCATCGTGGTGGGCA

 

CTGTCCTCATCACCCGAGGTCTGCGCTCAAGTGGGGCCTCCAGACACCAAGGGCCCTTGTGAGTCACACT

 

CCAGTAGGAAGGTGTACTGTCCATCTACAAGAACAGAAAAATGGACATACTAGATGACCTGGAACTATTT

 

TCTGGCCAAGTTCATCACGTACCTTTTCTCCTTCTGCCCTCCTCTGCTCACCTCTTCTCTGGGAAATAGG

 

ATGCTGTATCACCTCAGAGTTCACATATACCTCAGAGTTCTTGCCCTGACTATGTGATATTTCTTTCTCT

 

TCTCATCAGTTGCATAACATGGAATTGCTACGGTATTCCACCCAATAACCTAATCTCTAGTGACCCTGAT

 

CCCATGTCACCACGGAAGCAATAAATCCTTCTTTTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

 

we have to find...

 

 

 

a.Start codon (red)and Kozak sequence (blue).

 

b.Stop codon(red)

 

c.exon boundaries(yellow).

 

d.reading frame (+1, +2 or +3).

 

e.polyadenylation signal (not polyA tail)(green).

 

 

We we're told to use the NCBI blasting website to help.

 

 

If you could help with any of them, please let me know!!

 

 

 

 

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