Jump to content

Major Help Needed! How to determine Km (Michealis constant) in a biochemistry problem?

Angela1
in Biochemistry and Molecular Biology

Featured Replies

Greetings to all,

Question states: The cytochrome P450 system of liver in normal individuals has a capacity (Vm) to oxidize approximately 10 nmol of drug X per minute per gram of liver. When the concentration of drug X in the liver is 2um oxidation products are formed at the rate of 4 nmol/min/g of liver. What is the Km of cytochrome P450 for this drug?

 

Two answer choices were 5 nM or 3 uM. The correct answer is 3 uM.

 

I am totally lost on how to do this problem. Can someone please help?

 

 

Link

 

There is an explanation of how to find Km and you can rearrange the equations to find Km without drawing the graph.

Archived

This topic is now archived and is closed to further replies.

Go to topic listing

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications
Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.