simrankaur Posted September 16, 2011 Share Posted September 16, 2011 Hello.....I've been culturing human peripheral blood for karyotype analysis. However, in the past few days when i band my slides using giemsa and observe them under the microscope there is a cytoplasmic background that doesn't allow me to observe the chromosomes nicely and hinders the banding pattern. Can anyone tell me the reason why i am getting it and how do i get rid of this dense cytoplasmic background??? Secondly, there's splitting of chromosomes often seen while analysing the cells...any specific reason for splitting??? and how can it be avoided?? thanks in advance Link to comment Share on other sites More sharing options...
VSquared Posted May 11, 2012 Share Posted May 11, 2012 I would try giving the cells an extra wash in fixative, maybe two more, but I wouldn't do more than 3-5 total washes. That should alleviate the cytoplasm problem. As for the chromatids splitting, I'm not sure. We sometimes have that happen too, but I'm only a "year old" in this lab and I'm not sure why that happens yet. I know your question is from a while ago so I'm betting you already fixed your problem...? Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now