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Help with Flow?

beths
in Microbiology and Immunology

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Hi All,

 

I'm using flow to measure the size of my cells. I split the cells and get a pellet and resuspend them in a bath solution (140mM NaCl, 2.5mM KCl, 1.2mM CaCl2, 0.5mM MgCl2, 5mM Glucose and 10mM HEPES). However, when I measure the cells in Hypotonic solution (same as before but 75mM NaCl), the cells seem to be decreasing in size rather than increasing. Any ideas?

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