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The elution volume (Ve)

soconfused
in Chemistry

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hi guys!

 

i have difficulty in understanding this sentence, since English is not my language. here it is : "The elution volume (Ve) is the volume of buffer before which the peak of protein elutes." hallo! yes, i don't get it. does it mean Ve of that protein is the volume of the buffer before the UV-absorbance of that protein or does it mean that the volume of the buffer to the middle of the peak?

 

p.s! UV-absorbance and peak are different things here.

 

thanks for helping!

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