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Another calculation problem !


Gamewizard

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Hi,

 

I need some help on this type of question. How would I calculate this ?

 

If the absorbance at 540 nm of a sample of blood diluted 201 times in Drabkin's solution is 0.511 and that of a 0.572 g/L haemoglobin in Drabkin's solution is 0.325, what is the haemoglobin concentration of the blood sample in g/L?

(the answer should be to one decimal place)

 

I tried to work this out twice using the hb concentration formula which is absorbance of test*concentration of ref. prep. *dilution of blood/absorbance of ref. prep.

 

but the answers I got was incorrect, and I cant tell where i am going wrong so can someone please have a look at this. :(

 

Thanks

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