antiflux Posted March 19, 2011 Share Posted March 19, 2011 If in a subtilisin enzyme assay at pH=8.6 and 25C (both optimal), the absorbance of the solution increased by 0.43 @ 410nm in 1 min, what was the rate of the reaction expressed as Δ[product]/min? The diameter of the assay tube placed in the spectrophotometer was 1cm. I'm confused because the question doesn't give any concentrations, so how can i calculate the rate?? Link to comment Share on other sites More sharing options...
Greippi Posted March 19, 2011 Share Posted March 19, 2011 You have an absorbance increase - express the concentration as absorbance units. Link to comment Share on other sites More sharing options...
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