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chromatography problems

soconfused
in Biochemistry and Molecular Biology

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hi guys!

 

i am doing the chromatography. in scaling up i have to increase my volumetric flow rate but maintain my linear flow rate. my problem is i don't know how to calculate the column cross sectional area (cm2). i have asked the people in the math forum, but it seems like we get nowhere, so i hope you guys who work with this area can tell me how i can calculate it when the radius of the column is 2cm and the height is 5cm.

 

 

Linear flow rate (cm/hr) = volumetric flow rate (cm3/hr) /

column cross-sectional area (cm2)

 

 

 

thanks a bunch!

pi x r^2?

  • Author

hi skye!

 

are you sure column cross-sectional area (cm2) has this formula?

 

thanks!

Yep, the other thread seems to have better information. Even pictures.

  • 10 months later...

i think this is very primitive question.

volumetric flow rate= cs area*velocity

 

where c.s arae = pi/4*dia^2

 

i hope it will help you.

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