rotters Posted October 11, 2010 Share Posted October 11, 2010 Dear all , I have recently immunised some mice with a human tumour antigen in combination with Abisco adjuvant with a booster injection fours weeks after the first. I got a really good antibody response when testing via ELISA and have used the mouse spleens to isolate Vh and Vl chains from these animals. What I do not have any feel for is what sort of frequency (abundance) I can expect my antigen-specific B-cells to be in the total population of the cells I have isolated - could someone give me a rough idea? I know this is dependent on lots of variables but I really just wanted to have a general idea. Could I expect a frequency of 1% of total B-cells being antigen specific or would it be a couple of logs lower than that? I am not an immunologist (obviously!) and would really appreciate an expert opinion on this? Many thanks, Dominic Link to comment Share on other sites More sharing options...
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