can lacZ work when .....

[question]

if i insert lacZ into a gene and the translation gives a hybrid product with the sequences of both lacZ and this gene. i wonder if lacZ can still work and give blue colonies? and what about the function of this other gene?

 

 

wait so much for replies.

 

 

thanks so much in advance!

[badchad]

Might be a bit confused but I'll offer what I can.

 

Isn't lacZ used as a marker? That is, if the sequence of lac Z is "connected" to your gene sequence, the bacteria will first make your gene of interest, and then read through to make lacZ? if this is the case, everytime you insert lacZ into a gene, you'll get your gene, as well as lacZ.

 

Thus, if you got blue colonies, it would indicate your bacteria is translating the gene into which you inserted lacZ, but it wouldn't offer any insight into the function of the gene. Only that it's being expressed.

 

Could be completely wrong with this though