soconfused Posted September 10, 2004 Share Posted September 10, 2004 i am not sure if this is the right place to post this question, but since it is about proteins then here i go... about proteomics: the IEF dimension is performed using 7 cm or 18 cm IPG strips of selected pH range (3-10, 4-7, 3-6, 5-8, 7-10, 6-9, 3-7, 6-11, 3.5-4.5, 4-5, 4.5- 5.5, 5- 6, 5.5- 6.7). my question is what decides the selection of a specified pH range? i have looked for answers, but can't find any. it would help alot if anyone can tell me. thanks! wait for replies!!!! Link to comment Share on other sites More sharing options...
Skye Posted September 11, 2004 Share Posted September 11, 2004 The pH determines the isoelectrical point, where the proteins will settle. A range is needed that will encompass all the proteins in the sample, but also you'll want this to be as small as possible, so that the proteins are spread out across the gel, which will give better resolution. Link to comment Share on other sites More sharing options...
soconfused Posted September 11, 2004 Author Share Posted September 11, 2004 thanks! Link to comment Share on other sites More sharing options...
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