i am not sure if this is the right place to post this question, but since it is about proteins then here i go...

 

about proteomics:

 

the IEF dimension is performed using 7 cm or 18 cm IPG strips of selected pH range (3-10, 4-7, 3-6, 5-8, 7-10, 6-9, 3-7, 6-11, 3.5-4.5, 4-5, 4.5- 5.5, 5- 6, 5.5- 6.7).

 

 

my question is what decides the selection of a specified pH range? i have looked for answers, but can't find any.

 

it would help alot if anyone can tell me.

 

thanks!

 

wait for replies!!!!

The pH determines the isoelectrical point, where the proteins will settle. A range is needed that will encompass all the proteins in the sample, but also you'll want this to be as small as possible, so that the proteins are spread out across the gel, which will give better resolution.

thanks!