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high resolution melt for SNP analysis?

pedore
in Biochemistry and Molecular Biology

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Hi everyone,

 

I'm hoping someone here has used HRM to do SNPs. After reading some of the literature, I am a little confused about a few things, and if anyone can answer any of these I would very much appreciate it!

 

- can HRM only be used for one SNP at a time? Is there any way to multiplex?

 

- do you need special primers, or can you use any of the ordinary primers you can order online?

 

- is this really better than real time PCR to do SNPs? Speaking of real time PCR, can you do more than one SNP at a time that way either?

 

Thanks for any insight anyone has!

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