Jump to content

Yeast 2 Hybrid!

CoolScience
in Genetics

Featured Replies

I need help with my yeast 2 hybrid screen:( Can I get many false positive (blue) due to my own reagents?

I am looking for interactions between several genes. I have started by checking whether my Yeast strain auto-activated. And surprisingly it does! I have bought and tested few strains and all auto-activate! I am now questioning my X-Gal, LacZ, and filter/nitrocellulos papers! Can I get soooo many false positive because of my reagents, or it is true that my yeast auto-activate on their own?

your yeast should be autoactive to express BD-X, so when you fused your yeast with AD-Y plasmid can they interact (potentially) to activate (if they do interact) a third set of reporter.

Archived

This topic is now archived and is closed to further replies.

Go to topic listing

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications
Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.