Aisha_18 Posted October 21, 2009 Share Posted October 21, 2009 hi, I can really use some help. How would you convert ethanol substrate from 50 microlitres to micromoles/ unit? i dont know if this will help but the ethanol substrate was made as follows 1 mL of ethanol (95%v/v) was diluted with 199 mL of distilled water making the final concentration of the stock 100mM. 15 mL of this stock was thenplaced with 85mL of a buffer solution to make the final concentration 15mM. This stock was used for the enzyme assays. Link to comment Share on other sites More sharing options...
Horza2002 Posted October 22, 2009 Share Posted October 22, 2009 Given that, Moles = Mass / Molecular mass And Density = Mass / Volume Density = (Moles x Molecular mass) / Volume Therefore, Moles = (density x volume) / Molecular mass Once you have the number of moles of ethanol, you should be able to convert that into concentration easy enough Link to comment Share on other sites More sharing options...
Aisha_18 Posted October 23, 2009 Author Share Posted October 23, 2009 Thanks,but i am not sure what volume to use given my the dilutions. Do i just use the 1ml for the ethanol? Or just use whatever volume of the ethanol substrate i would have used. Link to comment Share on other sites More sharing options...
Al ameen Posted October 27, 2009 Share Posted October 27, 2009 you are to use 1mm of the ethanol. Link to comment Share on other sites More sharing options...
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