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Net optical density from a rate

Guest torontostudent
Guest torontostudent
in Biochemistry and Molecular Biology

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I have been using a method to calculate the activity of glucose-6-phosohate dehydrogenase.

 

I ran into a problem because my final activities are very low compared to results previously obtained.

 

I think I'm just doing the calculations wrong.

 

In a nutshell, I make up reactions in cuvettes and I measure the rates and I have to subtract certain rates from other rates. Then I'm supposed to use the "net optical density" from my subtracted rates.

 

How do I do calculate net optical density from a rate?

You measure the appearance of NADPH. Your blanc is: all components except your sample (enzyme), in some cases it is recomemnded to measure blanc, where substrate (G-6-P) is missing (NADP/NADPH is a cosubstrate in many reactions). So, where is the problem? I need more info!

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