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Tandem Repeats

ChazR
in Biochemistry and Molecular Biology

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We're planning on expressing a sequence that contains an insertion of two copies of a gene in a tandem repeat. Should we be concerned with recombination or 'slippage' and loss of a copy during cloning and expression in XL2-Blue/BL21 DE3 E. coli systems?

 

We have the option of using it from another organism, but it would be a eukaryote and we are concerned about codon optimization and poor expression.

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