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Measuring CoA levels in cultured cells


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Hi,

 

I am trying to measure CoA levels in HepG2 and Hek293 cells using a recycling enzymatic assay (Allred and Guy 1968). Has anyone done this as I am having difficulties obtaining reproducible results? Also I think I may have enough CoA in my samples to measure accurately.

 

Many Thanks,

 

Pascale

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