Good M Posted January 30, 2009 Share Posted January 30, 2009 Is there an immunohistochemistry antibody to assess monoclonality? For example antibodies to assess G6PD isoenzymes to see wether the cells I look at the microscope are monoclonal or policlonal. Please let me know. Link to comment Share on other sites More sharing options...
GDG Posted February 17, 2009 Share Posted February 17, 2009 What sort of cells are you looking at? There are probably MAbs specific for different G6PD isoenzymes available, but I'm not sure how they would distinguish between monoclonal cells and polyclonal cells. The terminology is usually applied to hybridoma cells (plasmacytoma cells crossed with B cells) that produce a particular antibody: one determines that the cells are monoclonal by the fact that they all produce the same antibody. Link to comment Share on other sites More sharing options...
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