Jump to content

What Protein Quantification Methods Are The Best?

Featured Replies

What protein quantification methods can be employed with enzimes? What are the basis and methodologies to be used for each case?(Please explain it to me as much as possible)

I've never used enzymes to quantify proteins. Instead I use colormetric assays that use stains such as Bradford Reagent or the BCA protein assay.

  • 3 weeks later...

Well, enzymes ARE proteins!!! After you determine a standard curve using Bradford or Lowry assays, you do a Western Blot; where you separate your proteins on a gel, transfer the proteins to a membrane which you then incubate with an antibody directed against your protein of interest. If you run a loading control of known concentration to check that you've loaded the same amount of protein per well, you can quantify your protein of interest. This of course is a hugely condensed version but look up Sambrook and Maniatis, the Bible, and it will guide you through it. I can send you more detailed instructions if you want via e-mail.

Archived

This topic is now archived and is closed to further replies.

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.

Configure browser push notifications

Chrome (Android)
  1. Tap the lock icon next to the address bar.
  2. Tap Permissions → Notifications.
  3. Adjust your preference.
Chrome (Desktop)
  1. Click the padlock icon in the address bar.
  2. Select Site settings.
  3. Find Notifications and adjust your preference.