I'm giving a lecture called "The Biochemists' Toolbox." My lecture will consist of the aspects of protein engineering (expression vectors, expression systems, and probing protein structure/function via mutagenesis). I'm having trouble finding the pioneers of this field. I'm well aware of PCR (Kary Mullis), however, I don't know where to look for the rest. Any suggestions? Any classical experiments in this field anyone's aware of?

I know Dr. George Georgiou from the University of Texas did a lot of work with directed evolution of proteins. Dr. Bill Degrado of University of Pennsylvania for his work in de novo design of proteins. I can't name much more than that off the top of my head.