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challenging questions.

olenka
in Biochemistry and Molecular Biology

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Hello I have a questions about isotopes and radioactivity.

 

I have red that that isotope of carbon 14C can be placed at any position in the glucose molecule and then , measurement of radioactivity in the products will indicate the metabolic fate of the radioisotope. So, if glucose is labeled in one or more positions with 14C, and fed to fermenting yeast, which form or forms of 14 C-glucose would give the most radioactivity in CO2 and the least ethanol???

 

My second question is about arsenate.

Arsenate is chemically similar to inorganic phosphate and is used by phosphate-requiring enzymes as an alternative substrate. However , inorganic arsenates are quite unstable and spontaneously hydrolyze. Arsenate is known to inhibit ATP production in glycolysis. Can somone explain me the mechanism of inhibition and identify the target enzyme please????

:confused::confused::confused::confused::confused::confused::confused:

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