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Does Ki value in binding experiments depend on Kd of radioligand?

paparatzi
in Biochemistry and Molecular Biology

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Hello,

 

I have a question with respect to the interpretation of Ki values from binding experiments:

 

In receptor binding assays Ki values are generated for compounds along with given Kd values of the used radioligands. As far as I understand does such an Ki describe the affinity of the investigated compound towards the receptor and should be independent of the used radioligand. Is this true? Or does the Ki value also depend on the radioligand and its affinity used in the binding assay?

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