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Help to interpretation of a figure

Lena
in Biochemistry and Molecular Biology

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Hi,

 

I have an exam this tuesday in enzyme kinetics and I have to go through an article and know how they did all the kinetic calculations.

 

My question is how they calculated Km and Kcat values from a figure showing a double reciprocal plot of elongation rate vs EF-Tu concentration

 

The article is 'ppGpp inhibition og elongation factors Tu, G and Ts during polypeptide synthesis' of Rojas, A-M., Ehrenberg, M., Anderson, S.G.E., and Kurland, C.G., Mol Gen Genet 1984 http://www.springerlink.com/content/m3m7250211t6g475/

 

It is fig 8 and the calculated Km and Kcat values are listed in table 2

 

I know this require that you'll spend some time reading the paper (or at least the part of the paper concerning fig 8 and table 2), but I really hope that someone would be kind enough to do this.. It would really help me (and my studying group)

 

Thank you for your time

 

- Lena

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